利用基因條碼鑑定蜂花粉來源植物之研究

Study on identification of bee pollen flora using DNA barcoding

徐培修(Pei-Shou Hsu) ； 羅時玟(Shi-Wen Luo)

《苗栗區農業改良場研究彙報》 10期 (2021/07) Pp. 61-79

摘要

本研究已建立分子鑑定蜂花粉來源植物之技術，以rbcL及trnH-psbA雙基因條碼對花粉DNA進行PCR增幅，與GenBank資料庫比對序列以鑑定植物種類。調查2020年春季南投縣名間鄉西方蜜蜂蜂場收集之蜂花粉，分離出17種蜂花粉，鑑定出16種植物，結果顯示rbcL單條碼種級鑑別力低，但trnH-psbA單條碼即可鑑定出多種植物。優勢粉源植物前三者為藿香薊（Ageratum conyzoides）、山黃麻（Trema orientalis）、大花咸豐草（Bidens pilosa var. radiata），重量佔比分別為21%、21%、12%。臺灣植物基因條碼資料庫的建立，應有助於蜂花粉分子鑑定應用技術之基礎。

關鍵字

基因條碼 ； 蜂花粉 ； 西方蜜蜂

並列摘要

In the present study, we investigated molecular identification technology of species origin for bee pollen pellets using rbcL and trnH-psbA multibarcoding to amplify pollen DNA, and to identify plant species by sequence aliments with GenBank database. The investigation was carried out in Apis mellifera apiary at Mingjian Township, Nantou County in 2020 spring. The bee pollen samples were collected from the apiary, 17 bee pollen pellets were separated, and 16 plant species were identified. It was suggested that rbcL alone has low species level taxonomic resolution, but trnH-psbA identified a variety of plant species. The top three dominant pollen source plants are Ageratum conyzoides, Trema Orientalis, and Bidens pilosa var. radiata, which account for 21%, 21%, and 12% by weight, respectively. Establishment of barcoding database for nectar sources is fundamental for application of molecular approaches to pollen identification in Taiwan.

並列關鍵字

gene barcoding ； bee pollen ； Apis mellifera