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基因改造農業貿易產品之取樣和檢測研究

Study on the Sampling and Detection of Genetically Modified Agricultural Trading Product

摘要


基改作物在國際企業間已廣泛流通,近年在安全性檢測和標示方面,各國均已制定相關標準與法規管理。為加速原物料進出口之流通手續,並增加檢驗之精確度,本研究以複式聚合酶鏈鎖反應,檢測基改大豆樣品。本研究設計三類型共20種取樣方法:類型Ⅰ是以不同取樣大小作一個比較,樣本數目佔整批種子的比例應達到多少才具代表性;類型Ⅱ採用階段性取樣,是由母本中先以較大數量取樣,當中再以較小數量取樣;類型Ⅲ於數批種子中以不同比例取樣,再混合成為複合樣本,比較複合樣品間彼此之差異,綜合各種取樣方法,以類型Ⅱ取樣方法12,其結果最接近總平均值。各種取樣和定量結果均無顯著差異性,因此在大宗國際基改物資報關之抽檢作業上可簡化採樣流程。

關鍵字

基因改造 取樣 檢測

並列摘要


Commercial trading of genetically modified agricultural product has been an important business in worldwide. In recent year, regulations and management policy on the safety and labeling of GM products were also been setup in many countries. To accelerate and enhance the accuracy on the sampling and detection of GM product during import and export trading, this study has evaluated factor such as sampling methods on the detection of trading products. Five events of GM soybean including Lectin and RRS (roundup ready) series specific gene were used. Twenty methods of sampling were developed which could be categorized into three types. Type I described different sampling size in whole community; type II is gradual sampling method; type III is a ratio sampling in different kind of samples. The result of study indicated that type II method showed to be the optimum method in mass sampling. It could be used to simplify sampling procedure in custom registration.

參考文獻


http://www.monsanto.com/monsanto/content/our_pledge/transparency/prod_safety/roundup_soybean/pss.pdf (Monsanto website), 2004
呂斯文(2005)。歐盟基因改造生物及其産品之技術指導綱領簡介。農政與農情。155,67-70。
Berdal, K. G.,Hoist-Jensen, A.(2001).Roundup Ready soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analysis.European Food Research Technology.213,432-438.
Brodmann, P. D.,Ilg, E. C.,Berthoud, H.,Hermann. A.(2002).Real-time quantitative polymerase chain reaction methods for four genetically modified maize and maize DNA content in food.Journal of the Association of Official Analytical Chemists.85,646-653.
Cochran, W. G.(1997).Sampling techiniques.New York:John Wiley.

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