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In-Vitro Culture of Leaf Primordium of Asplenium Nidus
並列摘要
The callus-like obstrusion had been formed from the surface of the leaf primordium of Asplenium nidus in the regulator-free MS medium after three-month cultivation. The reformed obstrusion would grow as double as the old one, when it was cut into many pieces and was put into 1/2MS liquid medium with two-week suspension culture. Then these calli were put into the MS semi-solid medium supplimented with 0.2~5mg/l BA. The shoot primordium would be formed directly from the surface of the explant after three-month cultivation in the MS semi-solid medium supplimented with 5mg/l BA. The new shoot primordia and calli would be formed and grown more and more, if the bud primordum was cut into several pieces and was cultured within the 1/2MS suspension medium about two weeks. Multiple adventitious buds and calli had been formed after cultivating these tissues in the MS or 1/2MS semi-solid medium supplimented with 0.2~5mg/l BA. The shoots formed in the 1/2MS semi-solid medium were better than in the MS medium, however shoots grown in the MS medium were better than in the 1/2MS semi-solid medium. The amount of shoots would be greatly formed in the MS medium supplimented with 5mg/l BA. Furthermore, it sould be much better for the shoots to grow in the medium supplimented with 250mg/l of casein hydrolysate.
延伸閱讀
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