- 期刊
- OpenAccess
Analysis Genetic Stability in Prunus humilis Bunge Plants after Cryopreservation Twice
並列摘要
Cryopreservation is one of the important biotechnologies. The Shoot-tips of Prunus humilis Bunge were successfully cryopreserved using vitrification method. Eight single-bud sibling lines were established as model system for genetic analysis. Amplified fragmentation length polymorphism (AFLP) assay was used to detect the variation of genetic stability. In the detected sites, only two sites where band pattern changed after cryopreservation, might be contribute to the change in DNA methylation status at EcoR Ⅰ recognition according to the different sequence following the recognition. About one third bands which had changed after cryopreservation twice seemed to have cumulative effect. Methylation sensitive amplified polymorphism (MSAP) assay was carried out for further investigation DNA methylation changes. A significant change in DNA methylation status induced by cryopreservation was detected. Variety of changes of band patterns could be classified to four kinds.
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