- 期刊
- OpenAccess
Comparison of Phytase Production by Aspergillus Ficuum under Submerged and Solid State Fermentation Conditions
並列摘要
Firstly, improvement of culture condition on phytase production by Aspergillus ficuum was investigated using submerged fermentation. The four factors studied were significantly effective on the phytase production. The optimum values for the factors were determined via response surface methodology (RSM) as: glucose, 5.23 g/100 mL; ammonium sulphate, 1.6 g/100 mL; wheat bran, 3.28 g/100 mL, and fermentation time, 198.30 h. The produced amount of phytase under these conditions was 40.21 U/ mL. In the next stage, the process was scaled up to a 3-L batch bioreactor. The highest level of phytase produced in this stage was 85.41 U/mL, which resulted in a 2.1-fold enhancement. In the last stage, the results of optimization of phytase production in solid-state fermentation were further scaled up to packed bed solid-state bioreactor. The highest level of phytase produced in this stage was 87.75 U/gds, which resulted in a 3.4-fold enhancement.
延伸閱讀
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