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Isolation, Sequence Identification and Tissue Expression of a Novel Tobacco (Nicotiana Tabacum) Gene‐GME1
並列摘要
The complete coding sequence of one tobacco (Nocotiana tabacum) gene‐GDP‐mannose 3', 5'‐epimerase (GME1), was amplified based on two tobacco ESTs. The complete coding sequence of tobacco GME1 gene was 1,131bp which encodes a protein of 376 amino acids. Sequence analysis revealed that the GME1 of tobacco shares high homology with the GME1 of lycopersicon esculentum (96%), peach (95%), castor bean (94%), malpighia glabra (94%), ribes nigrum (Accesession number: ACM90324, 93%), cucumber (92%), camellia sinensis (92%), soybean (92%). The expression profile was studied and the results indicated that tobacco GME1 gene was differentially expressed in detected tobacco tissues including leaf, stem, root and flower.
延伸閱讀
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