馬來絲狀蟲之蚊子感染

Experimental Infection of Mosquito with Subperiodic Filarial Brugia Malayi

吳金正(Chin-Cheng Wu) ； 范秉真(Ping-Chen Fan) ； 何兆美(Chau-Mei Ho)

《檢驗及品保雜誌》 2卷2期 (2013/05) Pp. 94-100

https://doi.org/10.6573/JTQA.201305_2(2).06

摘要

為了瞭解馬來絲狀蟲對各種蚊子之感染能力，吾等選擇6屬16種蚊子：埃及斑蚊（利物浦族）（Aedes aegypti）、白點斑蚊（Aedes albolineatus）、白線斑蚊（Aedes albopictus）、日本斑紋（Aedes japanicus）、馬氏斑紋（Aedes malikuli）、東鄉斑紋（Aedes togoi）、四斑瘧蚊（Anopheles quadrimaculatus）、中華瘧蚊（Anopheles sinensis）、斯氏瘧蚊（Anopheles stephensis）、黃色叢蚊（Armigeres flavus）、白腹叢蚊（Armigeres subalbatus）、海岸家蚊（Culex alis）、熱帶家蚊（Culex quinguefasciatus）、三斑家蚊（Culex tritaenicrchynchus）、斑腳沼蚊（Monsonia uniformis）及竹生翠蚊（Tripteroides bambusa）分別吸取受染多夜間定期出現馬來絲狀蟲之大白鼠、黑白鼠或沙鼠（血絲蟲密度約10~20/cm2）。1,724隻吸血蚊子經過10~14天飼養後在解剖顯微鏡下分別予以解剖，共檢查1,254隻，在5種122隻蚊體中均檢獲第三期（感染性）幼蟲，感染率以斑腳沼蚊（62.5%）最高，次為東鄉斑蚊（38.3%）、中華瘧蚊（37.5%）及埃及斑蚊（35.4%），白腹叢蚊僅１隻（0.3%）感染。本實驗證明斑腳沼蚊、中華瘧蚊及埃及斑蚊（利物浦族）均為媒介多夜間定期出現馬來絲狀蟲之良好宿主。反之，白腹叢蚊及其它未感染之11種蚊子，對此種絲狀蟲均具有抗菌性。再者，中華瘧蚊第一次被證明其為馬來絲狀蟲之媒介蚊蟲，傳播多在夜間定期出現。

關鍵字

馬來絲狀蟲 ； 蚊子感染

並列摘要

To realize the susceptibility of mosquitoes to subperiodic filarial Brugia malayi, 16 species belonging to 6 genera were studied in the laboratory. These mosquitoes included Aedes (Ae.) aegypti, Ae. Albolineatus, Ae. albopictus, Ae.. japonicas, Ae malikuli, Ae. Togoi, Anopheles (An.) quadrimaculatus, An. sinensis, An. stephensis, Armygerus (Ar.) flavus, Ar. subalbatus, Culex (Cx.) alis, Cx. quinquefasciatus, Cx. tritaeneiorhynchus, Mansonia (Ma) uniformis, and Tripteroides bambusa. Totally 1,724 female adults of the mosquitoes were permitted to feed on the rats or gerbils that had been infected with subperiodic filarial B. malalyi. These rats or gerbils were found to be loaded with the microfilaria (mf) of B. malayi at the density of 10~20 mf/cm2 approximately in peripheral blood. Ten or fourteen days after blood feeding 1,254 mosquitoes were finally dissected and examined the development of microfilaria. The third-stage infective larvae of B. malayi were recovered from 122 mosquitoes in 5 species, namely Ma. uniformis, Ae. togoi, An. sinensis, Liverpool strain Ae. aegypti and Ar. subalbatus. The susceptibility for these 5 species were 62.5%, 38.3%, 37.5%, 35.4% and 0.3%, respectively. Conclusively, the first 4 species, Ms. uniformis, Ae. togoi, An. sinensis and Liverpool strain of Ae. aegypti are considered as a good vector for B. malalyi. On the other hand, Ar. subalbatus is a poorly efficient vector in the transmission of this parasite.

並列關鍵字

Brugia malayi ； mosquitoes ； laboratory infection