初步評估沙增管（Salmonella enricher）及沙測管（Salmonella checker）組合快速且高通量地篩檢沙門氏菌（Salmonella spp.）

Preliminary Evaluation of Combined Salmonella Enricher and Checker for Rapid and High-Throughput Screening of Salmonella spp.

陳亭蓁(Ting-Chen Chen) ； 吳尚宜(Shang-Yi Wu) ； 許惟婷(Wei-Ting Hsu) ； 賴宣羽(Hsuan-Yu Lai) ； 蔡文城(Wen-cherng Tsai)

《檢驗及品保雜誌》 9卷1期 (2020/01) Pp. 1-9

https://doi.org/10.6573/JTQA.202001_9(1).01

摘要

沙增管（Salmonella enricher）及沙測管（Salmonella checker）組合為啟新生物科技有限公司針對肉品中沙門氏菌（Salmonella spp.）篩檢而生產的一種簡易操作裝置。本研究之目的為測試此組合偵測各種非傷寒型沙門氏菌的效能，評估項目包括最低偵測極限及Salmonella enricher之最適當增菌時間。本研究以5亞種／株能使人類與冷血動物致病的Salmonella spp.作為測試菌，分別為Salmonella typhimurium （ATCC 13311）、S. enterica subsp. houtenae （ATCC 15783）、S. choleraesuis （ATCC 13076）、S. enterica subsp. salamae（ATCC 6959）及S. enterica subsp. diarizonae （ATCC 12325）。分別接種每一測試菌純菌的系列稀釋液至組合中，結果顯示此檢測組合對上述5種個別測試菌／株之最低偵測極限均為10 CFU。將5種沙門氏菌菌種／株分別與31種其它菌種以同等菌量（10^5 CFU）混合，初步評估結果指出Salmonella enricher增菌8至17小時移種Salmonella checker為最適當時間，若移種早於8小時或遲於20小時（含）將影響沙門氏菌在Salmonella checker的檢出（呈黑色）。總之，初步評估結果指出本檢測沙門氏菌組合是值得採用之一種方便且簡易的高通量檢測食品中非傷寒型沙門氏菌裝置，爾後將應用此套組於家禽或家畜屠體沖洗液之沙門氏菌檢測以探討其實用效能。

關鍵字

沙增管 ； 沙測管 ； 最低偵測極限 ； Salmonella enricher移種之最適當時間

並列摘要

The combined Salmonella enricher and checker is a simple operation device manufactured by Creative Biotechnology Co., Ltd. (New Taipei City, Taiwan) for screening Salmonella spp. in meat products. The purpose of this study was to determine its efficacy in the detection of various nontyphoidal Salmonella species. Items evaluated included the minimum detection limit and the optimal duration for enrichment. Five subspecies or strains of Salmonella spp., which can cause diseases in humans and/or cold-blood animals, were used as test bacteria, including Salmonella typhimurium (ATCC 13311), S. enterica subsp. houtenae (ATCC 15783), S. choleraesuis (ATCC 13076), S. enterica subsp. salamae (ATCC 6959), and S. enterica subsp. diarizonae (ATCC 12325). Each of series diluted suspensions of five test bacterial species was inoculated to Salmonella enricher and checker, individually. Results showed that the minimum detection limit of each of these bacterial species with this device was 10 CFU. Each test sample was a suspension of five test bacterial species mixed with 31 other non-Salmonella species at the concentration of 10^5 CFU each. Results showed that the optimal duration for enrichment in the Salmonella enricher before transferring the sample to the Salmonella checker of the device was 8 to 17 hours. An enrichment time less than 8 or greater than 20 hours resulted in poor results. In conclusion, results of this study indicate that the combined Salmonella enricher and checker is a convenient and simple device for high-throughput detection of nontyphoidal Salmonella spp. in food. In the future, this device will be used to detect Salmonella spp. in poultry, livestock carcass washings, or raw fish (e.g., sashimi) to further determine its usefulness.

並列關鍵字

Salmonella enricher ； Salmonella checker ； the minimum detection limit ； optimal duration for enrichment in Salmonella enricher