具有調節單胺類神經傳遞物質（Monoamine Neurotransmitter）能力的新穎精神益生菌Levilactobacillus（Lactobacillus）brevis GKJOY

A Novel Psychobiotic-Levilactobacillus (Lactobacillus) brevis GKJOY Capable of Modulating Monoamine Neurotransmitter

陳彥博(Yen-Po Chen) ； 林詩偉(Shin-Wei Lin) ； 陳炎鍊(Yen-Lien Chen) ； 邱駿紘(Chun-Hung Chiu) ； 陳勁初(Chin-Chu Chen)

《檢驗及品保雜誌》 10卷2期 (2021/04) Pp. 92-98

https://doi.org/10.6573/JTQA.202104_10(2).06

摘要

近來研究發現腸道微生物能夠產生人腦中發現的大多數神經傳遞物質（neurotransmitter），越來越多的證據支持腸道微生物影響中樞神經行為，單胺類（monoamine）神經傳遞物質被認為與精神疾病發展有關，包含憂鬱症（depression）、精神分裂症（schizophrenia）等。本研究從天然發酵醃製的泡菜產品中取樣，篩選出一株具有高GABA（γ-Amino butyric acid）產量的菌株，Levilactobacillus（Lactobacillus）brevis（短乳桿菌）GKJOY。於動物實驗中，每天管餵小鼠1×10^9 CFU劑量的L. brevis GKJOY益生菌，持續四個星期。服用此菌兩週後，每天一次將小鼠固定在禁錮器（restrainers）中兩小時，持續兩週。與對照組相比，服用L.brevis GKJOY四周後，大腦中多巴胺（dopamine）與血清素（serotonin）的含量顯著提升（p ＜0.05）。結果指出長期服用益生菌L. brevis GKJOY將可改善憂鬱症引起腦部單胺類低下問題，即提高腦中單胺類傳遞物。

關鍵字

憂鬱症 ； GABA ； 多巴胺 ； 血清素

並列摘要

Some human gut bacteria are known to produce neurotransmitters. Monoamine neurotransmitters are the most important ones with effects on the pathophysiology of mood disorders, such as depression and schizophrenia. The bacterium Levilactobacillus (Lactobacillus) brevis GKJOY isolated from traditionally pickled and fermented cabbage was found to produce high amounts of gamma aminobutyric acid (GABA). In this study, the effect of L. brevis GKJOY on depression was examined using an animal depression model. Mice were fed with L. brevis GKJOY at 1.0×10^9 CFU/mouse/day for two weeks and then immobilized for two hours with a restrainer every day for two weeks to cause depression with continual L. brevis GKJOY feeding. Compared with the control mice without L. brevis GKJOY feeding, the treated mice were found to have higher levels of dopamine and serotonin in the brain after four weeks of treatment (p＜ 0.05). These results suggest the potential of using L. brevis GKJOY as probiotics can improve of low monoamines caused by depression, that is, inceease monoamine transmitters in the brain.

並列關鍵字

Depression ； GABA ； dopamine ； serotonin