從牛樟芝（Antrodia cinnamomea）菌絲體萃取的純化4-乙醯基蒽醌B（4-AAQB）具有抑制腦膜癌循環腫瘤細胞的潛力

Inhibitory Effect of Purified 4-Acetylantroquinonol B Form Antrodia cinnamomea Mycelium on Meningeal Carcinoma Cells

呂庭宇(Ting-Yu Lu) ； 李宗儒(Tsung-Ju Li) ； 朱心彤(Hsin-tung Chu) ； 莊東岳(Tung Yueh Chuang) ； 陳勁初(Chin-Chu Chen)

《檢驗及品保雜誌》 11卷3期 (2022/07) Pp. 106-115

https://doi.org/10.6573/JTQA.202207_11(3).04

摘要

腦瘤的發病率為每10萬人中有4-5例，致死率高且預後差。本研究中，吾等從惡性腦膜瘤患者的血液中獲得循環腫瘤細胞，並且以牛樟芝（Antrodia cinnamomea）菌絲體之純化化合物：4-Acetylantroquinonol B（4-AAQB）測試其對這種細胞的抑制作用。結果顯示，4-AAQB在4μg/mL的濃度下對循環腦膜腫瘤細胞的生長有約50%的抑制作用，在20μg/mL的濃度下則有65%。結果亦顯示，4-AAQB和腦膜癌治療藥物Lomustine的併用並沒有協同作用。另外，還發現，4-AAQB可抑制LPS誘導的單核細胞產生一氧化氮。綜上所述，本研究結果指出，從牛樟芝菌絲體中純化的生物活性化合物4-AAQB具有抑制腦膜癌生長的潛力。

關鍵字

腦膜癌（Malignant meningioma） ； 牛樟芝（Antrodia cinnamomea） ； 循環腫瘤癌細胞（circulating tumor cells） ； 4-Acetylantroquinonol B（4-AAQB）

並列摘要

Brain tumors have an incidence of 4-5 cases per 100,000 people with a high fatality rate and poor prognosis. In this study, we obtained circulating tumor cells from patients with malignant meningioma and tested whether the purified compound, 4-Acetylantroquinonol B (4-AAQB), from Antrodia cinnamomea mycelium has inhibitory effect on these cells. Result showed that 4-AAQB inhibited the growth of circulating meningeal tumor cells by approximately 50% at the concentration of 4 μg/mL and 65 % at 20 μg/mL. Our results also showed that combined usage of 4-AAQB and Lomustine had no synergistic effect.We also found that 4-AAQB inhibited LPS-induced production of nitric oxide by monocytes. Taken together, results of this study suggest that the purified bioactive compound, 4-AAQB, from A. cinnamomea mycelium has the potential to inhibit the growth of meningeal carcinoma.

並列關鍵字

Malignant meningioma ； Antrodia cinnamomea ； Circulating tumor cells ； 4-Acetylantroquinonol B (4-AAQB)