酵素連接免疫分析法於組合化學之應用

Using Enzyme-Linked Immunosorbent Assay for Combinatorial Screening

楊靜依(Ging-Yi Young) ； 朱延和(Yen-Ho Chu)

《化學》 58卷1期 (2000/03) Pp. 173-176

https://doi.org/10.6623/chem.2000017

並列摘要

One of the many challenges in drug discovery process is to identify molecules from millions of compounds that bind tightly to biological target. This step usually involves screening libraries with targetspecific functional (cell-based or in vitro biochemical) assays and synthesizing structural analogs to optimize these leads. Many biochemical assays including scintillation proximity assay, fluorescence polarization, colorimetric assay, membrane permeation assay, affinity capillary electrophoresis, solid phase assay, and enzyme-linked immunosorbent assay (ELISA) have been successfully developed . In this report, we describe the principle of ELISA and its applications to combinatorial chemistry.

並列關鍵字

Enzyme-Linked Immunosorbent Assay ； Combinatorial Library Screening