電化學感測器分析有機磷與氨基甲酸鹽農藥殘留

利用有機磷(organophosphorous)與氨基甲酸鹽(carbamate)類農藥對乙醯膽鹼酯酵素(acctylcholinesterase, AChE)活性抑制之原理，在流動注入分析系統中，使用固定化AChE反應器與膽鹼氧化酵素(choline oxidase, ChOx)及辣根過氧化酵素(horseradish peroxidase, HRP)碳粉電極共同組成之電流式生物感測器，偵測巴拉奧克松(paraoxon)與加保扶(cabofuran)等農藥殘留。本系統為自動化操作，再現性佳，分析一次時間只需12分鐘，並使用一較不同的設計來避免再活化過程對ChOx可能產生的不良影響。使用不同來源AChE（分別取自蒼蠅頭及電鰻）進行偵測，前者分析巴拉奧克松及加保扶皆有較低的偵測極限(10ppb及23ppb)，且在8～100ppb間有良好的線性關係，比較不同固定化電鰻AChE反應器(CPG及Nylon-6 tubing)分析結果，Nylon-6 tubing反應器分析巴拉奧克松可得較低偵測極限(63ppb)，且在50～300ppb間有良好的線性關係。本系統對真實樣品的分析結果與標準品相當吻合，顯示適合環境分析等方面的應用。

關鍵字

有機磷農藥；氨基甲酸鹽類農藥；乙醯膽鹼酯酵素；膽鹼氧化酵素；辣根過氧化酵素；蒼蠅頭

並列摘要

A carbon paste electrode modified with choline oxidase (ChOx) and horseradish peroxidase (HRP) was designed as the amperometric biosensor for organophosphorous pesticides (OPPs) determination. The OPPs was measured based on the inhibition of immobilized acetylcholinesterase (AChE) as a reactor mounted before the paste electrode in the continuous flow system. Two different sources of AChE from eel and house fly head were studied respectively. The better detection limit for paraoxon (10ppb) and carbofiran (23ppb) was found in the latter AChE reactor with a linear range of 8-100ppb. Two different type of AChE reactors (CPO and Nylon-6 tubing) were studied also. The better detection limit for paraoxon (63ppb) was found in the latter AChE reactor with a linear range of 50-300ppb. A new design was developed to prevent the adverse effect of ChOx during AChE reactivation process. This system is fully automatic and the throughput of 5 sample h^(-l) is obtained with a good reproducibility. The correlated data from spiked river water and fruit juice real sample shows the feasibility of this system for environmental application.

並列關鍵字

choline oxidase(ChOx) ； horseradish peroxidase(HRP) ； amperometric biosensor ； organophosphorous pesticides(OPPs) ； acetylcholinesterase (AChE) ； fly head

國際替代計量

電化學感測器分析有機磷與氨基甲酸鹽農藥殘留

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