生理學於蛋白質體學的應用

Application of Physiology in Proteomic Studies

蔡美玲(Mei-Ling Tsai)

《化學》 61卷2期 (2003/06) Pp. 291-296

https://doi.org/10.6623/chem.2003030

摘要

面對物理，化學刺激，生理功能須藉著短期或長期之調控予以調適。一般而言，短期生理調控常因蛋白活化；長期則因結構或功能性蛋白生成或基因的活化影響組織或細胞重塑。因此目前之功能性蛋白質體之研究著重於長期調控後的結果。雖然如此，對於少量具功能性之蛋仍有偵測上之限制。因此仍須結合傳統之蛋白濃縮之技術與以改善。就目前已知蛋白修飾之作用包括磷酸化、醣基化、乙醯化、脂肪化或是氮基化。未來若能結合蛋白修飾之概念與蛋白質體之分離與鑑定必能改善分離與純化之困境，並且為短期生理調控之研究提出新的出路。

關鍵字

蛋白修飾 ； 磷酸化 ； 醣基化 ； 乙醯化 ； 脂肪化 ； 氮基化

並列摘要

In response to physical or chemical stimuli, physiological functions can be modified by short- or long-term regulation. The former requires the activation of proteins by post-translational modification and the latter requires the induction of newly synthesized proteins. Therefore, functional proteomic studies are suitable for exploring the proteins or peptides involved in long-term regulations but are not for those involved in short-term regulations. Even though functional proteomics can be used in studying the proteins involved in long-term regulations, detection of small amount of functional proteins is still limited. Therefore, conventional methods of protein concentrations may be applied to improve resolution. Current understanding of post-translational modification includes phosphorylation, glycosylation, lipidation, and nitration. In the future, the application of post-translational modification to proteomics may improve current pitfalls on separation/purification and further explores the proteins involved in short-term regulations.

並列關鍵字

post-translational modification ； phosphorylation ； glycosylation ； nitration ； lipidation ； aceylation