Luminol化學發光之增強及應用

Luminol 氧化產生之化學發光通常在鹼性溶液及催化劑存在下進行，任何有利於主要中間體luminol自由基及luminol過氧化物之形成以及增加激態放光體之產生效率和量子產率之因素都能夠增強其化學發光。多羥酚、酚酸、苯硼酸可以更有效率地與過氧化酵素中間體作用產生自由基，進而大量形成luminol自由基，因此能顯著增強化學發光。Guanidine hydrochloride、碳酸鈉、氯化鈉能加速微過氧化酵素中間體的形成，使其能更快產生luminol自由基而增強化學發光。許多還原試劑可以和溶氧或其他氧化劑作用產生超氧離子、增強劑自由基、氫氧自由基、單態氧、或其他自由基及活性物質而更迅速的產生luminol自由基以增強化學發光。金屬螯合物、金奈米粒子的表面都能更有效催化的luminol氧化而增強化學發光。微胞及螢光接受體可提升發光系統之反應速率、激發效率及量子產率，也能增強化學發光。化學發光增強系統的應用極為廣泛，可偵測參與反應之各物種包括化學發光試劑、氧化劑、催化劑、增強劑、抑制劑及其他會影響化學發光訊號的物質。

關鍵字

化學發光；增強效應

並列摘要

Chemiluminescence (CL) from the oxidation of luminol is usually carried out in an alkaline solution and in the presence of a catalyst. Any factors that facilitate the formation of the key intermediates luminol radical and luminol endoperoxide as well as increase the excitation efficiency and quantum yield of the emitter will enhance the CL emission. Polyhydroxy phenols, phenolic acids and phenyl boronic acids react efficiently with the intermediates of peroxidases to form radicals, thereby increasing the production of luminol radical for enhanced CL emission. Guanidine hydrochloride, sodium carbonate and sodium chloride accelerate the formation of intermediates of microperoxidases, which in turn leads to a fast production of luminol radical for enhanced CL. A variety of reducing agents react with the dissolved oxygen and other oxidants to form superoxide anion, enhancer radical, hydroxyl radical, singlet oxygen and other radicals or reactive species to enhance the production of luminol radical and CL emission. Metal chelate and gold nanoparticles efficiently catalyze the oxidation of luminol for enhanced CL. Micelles and fluorescence acceptors increase the rate of CL reaction, the excitation efficiency and quantum yield of CL emitter for enhanced CL. Any species that involves in the CL reaction, including CL reagent, oxidant, catalyst, enhancer and inhibitor, can be determined by the enhanced CL technique.

並列關鍵字

Luminal ； chemiluminescence ； enhancement

國際替代計量

Luminol化學發光之增強及應用

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