液相層析／質譜儀的基質效應與因應之道

Matrix Effects on Liquid Chromatography/Mass Spectrometry and How to Tackle the Problems

陳家揚(Chia-Yang Chen)

《化學》 65卷2期 (2007/06) Pp. 151-155

https://doi.org/10.6623/chem.2007017

摘要

樣本中的基質成分可能改變游離源離子化的效率，尤其是抑制離子的形成，造成偵測靈敏度的下降，以及影響分析的準確度與精密度。由於質譜儀，特別是串連式質譜儀，具有高靈敏度和高選擇性，加以造成干擾的基質常常未被離子化而未出現於質譜圖，往往造成分析者對於基質效應的忽略。本文簡介基質效應對於定量的影響，如何加以評估，並探討各種常用來降低或移除基質效應的方法其優缺點和適用性。

關鍵字

基質效應 ； 離子抑制 ； 電灑游離 ； 大氣壓化學游離 ； 同位素稀釋技術

並列摘要

Matrix components in the extract may influence the ionization efficiency of an analyte, and in most cases, suppress the formation of ions. This will deteriorate the sensitivity, accuracy, and precision of the analysis using liquid chromatography/mass spectrometry. Because a mass spectrometer, especially a tandem mass spectrometer, possesses unparalleled sensitivity and selectivity, and many of the matrix components are unseen because of not ionized, the issue of matrix effects is usually overlooked. This article introduces the aspects of matrix effects, the methods for evaluating them, and the solutions to the relating analytical problems.