找尋生物指標的利器：差異蛋白質體學分析平台之簡介

Powerful Tools for Biomarker Discovery: An Overview of Differential Proteomics

陳頌方(Sung-Fang Chen) ； 黎培鈺(Pei-Yu Li) ； 沈蕙如(Hui-Ju Shen)

《化學》 65卷2期 (2007/06) Pp. 211-224

https://doi.org/10.6623/chem.2007022

摘要

差異蛋白質體分析技術持續在找尋蛋白質生物指標分子上占舉足輕重的地位，並且在生物指標的開發上具先導的位置。本文著重於介紹現今被廣泛應用的差異蛋白質體分析平台，這些平台通常涉及凝膠電泳或是液相層析做為分離的工具，同時搭配同位素標記與質譜分析達到定性與定量的目的。此外，各平台在概念或實施方法上各有其優缺點。差異蛋白質體分析平台的最終目標在於發掘、鑑定在不同生理、病理狀態下參與的蛋白質標的分子。最後，成功的生物指標開發不單只靠找尋生物指標，尚有生物指標驗證等工作；且結合其他學科智識，生物指標開發之臨床應用是可以高度期待的。

關鍵字

生物指標 ； 差異蛋白質體學 ； 質譜 ； 蛋白質體

並列摘要

Differential proteomic techniques continue to be pivotal in biomarker discovery, and serve as a leading role in biomarker development. In this article, several widely recognized differential proteomic platforms are surveyed. These platforms usually involve either gel electrophoresis or liquid chromatography for separation, and combine isotope labeling with mass spectrometric analysis for identification and quantification. Furthermore, each approach has advantages and disadvantages from the conceptual and the methodological viewpoints. The ultimate goal of differential proteomics is to discover and to identify protein marker in different physiological or pathological states. A successful biomarker development not only depends on biomarker discovery but also relies on biomarker validation. Incorporating knowledge from other disciplines, biomarker development for useful and valid clinical applications is highly expectable.

並列關鍵字

Biomarker ； Differential proteomics ； Mass spectrometry ； Proteome ； DIGE ； cICAT ； iTRAQ ； SILAC ； SELDI