摘要
脲酶(urease)是第一個被結晶出來的蛋白質，這個發現使得酵素學的研究有了極大的變革和進展，此酵素也是第一個被證實其活性中心是由鎳金屬離子所組成，可以快速水解尿素產生氨氣與二氧化碳。除了在植物與土壤的細菌中可找到脲酶的蹤影，亦可在使人患有消化性潰瘍之幽門螺旋桿菌中發現它的存在，此細菌存活在病患的胃幽門內壁黏膜中，會造成嚴重的腸胃疾病。目前許多科學家試著利用化學方法來合成可模擬脲酶雙鎳活性中心之擬態化合物，並進行該化合物水解尿素的反應，期望能經由這些相關研究來更瞭解脲酶催化尿素水解的反應機制並提供因幽門螺旋桿菌所造成之腸胃疾病的醫治方法。
並列摘要
Urease is the first crystallized protein, which dramatically changed the methodology and development of enzymologic studies. It has also been confirmed to be the first nickel dependent enzyme with a dinuclear active site, which can rapidly catalyze the hydrolysis of urea to form ammonia and carbon dioxide. Urease can be found not only in plants or soil bacteria, but also in Helicobacter Pylori, which causes gastric inflammation and severe peptic ulcer disease, and survives in the gastric mucosa of patients. Currently, scientists are attempted to prepare model complexes to mimic the dinickel active site of urease through chemical synthesis, and study the reactivity of the prepared complexes towards the hydrolysis of urea. By such investigations, we hope to further understand the hydrolytic mechanism of urea catalyzed by urease, and prove a treatment for curing peptic ulcer disease caused by Helicobacter Pylori.
延伸閱讀
- Tien, P. C. (2012). 黏菌中與LARGE相似的N-乙醯葡萄糖胺轉移酶(DdGnT-15)的生物化學功能研究 [master's thesis, National Taipei University of Technology]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0006-2908201210562500
- 賴彥錫（2017）。磺酸化電活性聚脲之製備、鑑定及其在防腐蝕塗料之應用研究〔碩士論文，中原大學〕。華藝線上圖書館。https://doi.org/10.6840/cycu201700865
- 黃致鈜（2013）。Orpinomyces sp. Cel6葡聚醣水解酶之結構與功能分析〔碩士論文，國立臺北科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0006-2008201314382900
- 陳勻錡（2012）。The Improvement of Efficiency in the Lysozyme Renaturation Process〔博士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2012.10132
- 謝東宏（2004）。Synthetic effort on the development of activity probes for glycosidases〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2004.02200