含銅亞硝酸還原酶及其生化擬態化合物之歷史回顧及展望

Research Developments on the Copper Nitrite Reductases and Its Biomimetic Complexes

張育綸(Yu-Lun Chang) ； 莊宛蓉(Wan-Jung Chuang) ； 卓詩為(Shih-Wei Chuo) ； 許智能(Sodio C. N. Hsu)

《化學》 69卷2期 (2011/06) Pp. 119-133

https://doi.org/10.6623/chem.2011012

摘要

亞硝酸還原酶在厭氧呼吸過程的脫氮細菌中扮演相當重要的角色，整個過程最終會將亞硝酸根轉變成一氧化氮氣體。銅亞硝酸還原酶會催化其反應，NO_2- + 1e- + 2H^+ → NO + H_2O。銅亞硝酸還原酶的蛋白質晶體結構顯示，亞硝酸還原酶活性中心包括藍綠色型一銅，其負責電子傳遞，以及負責催化的型二銅。從一些酵素結構及模型化合物所推測的反應機制顯示，整個反應過程包含一個重要的中間產物 {Cu-NO}^(10)，此中間產物的分離將有助於科學家們更加了解銅亞硝酸還原酶的反應機制。

關鍵字

銅 ； 一氧化氮 ； 亞硝酸根

並列摘要

Nitrite reductase (NiR) is a key enzyme in the anaerobic respiratory pathway of denitrifying bacteria in which nitrite is eventually converted to nitric oxide gas. Copper nitrite reductases catalyse the reaction, NO_2^- + 1e^- + 2H^+ → NO + H_2O. The protein crystallographic study of the nitrite reductase enzyme reveals the locale of the type 1 copper blue-green electron-transfer center and the type 2 copper catalytic center in NiR. Several catalytic mechanisms base on the enzymes and model compounds study are discussed and reveal an important intermediate which may assign to be a {Cu-NO}^(10) complexes. The isolation of the {Cu-NO}^(10) intermediate will help scientist to gain more fundamental knowledge for a deeper understanding of copper nitrite reductase enzymes.

並列關鍵字

Copper ； Nitric oxide ； Nitrite