奈米混成無機層材對蛋白質之吸脫附機制的探討

Study on Adsorption and Desorption Behaviour of Proteins by Nano Inorganic Layered Materials

徐兆稹(Chao-Chen Hsu) ； 詹婕(Chieh Chan) ； 蔡宗燕(Tsung-Yen Tsai)

《化學》 70卷1期 (2012/03) Pp. 25-30

https://doi.org/10.6623/chem.2012003

摘要

本研究利用天然黏土(具有可置換陽離子型)作為蛋白質吸附與脫附的載體，探討天然黏土與有機改質黏土的本質差異上，分別對於蛋白質的吸附與脫附的機制是否有不同的效果，天然黏土雜質的含量雖多，但是其價格低廉，可藉由適當之純化步驟來改善降低天然黏土雜質的含量，純化之後的天然黏土可成為良好之吸附材，利用離子交換法可與蛋白質吸附/插層及脫附，且其高視徑比的結構特性，能與蛋白質有更多的接觸面積，因此將天然黏土添加於牛血清蛋白質(BSA)，紅螢光蛋白(RFP)中，藉由改變pH值使蛋白質所帶的電荷性質改變，得以與層狀材料之層間離子進行離子交換反應，並藉由探討其吸脫附的行為，最後期許此分離技術獲得之高純度螢光蛋白，應用在高單價藥物的篩選、基因療法上病毒載體的評估、生物病蟲害上的防治及進行生物感測晶片元件的製作。

關鍵字

蒙脫土 ； 牛血清蛋白 ； 螢光蛋白 ； 吸附 ； 脫附

並列摘要

In this work, natural clays, with cation exchange capacity (CEC), and synthetic layered double hydroxides (LDH) inorganic materials, with anion exchange capacity (AEC), would be applied as the templates for the mechanism study of the protein adsorption and desorption. In order to understand how pure the proteins could be performed by this new separation technology through the different templates, which contain different (1) composition, (2) aspect ratio, and (3) crystal defect, several types of natural clays and synthesized LDHs will be the starting materials, which will be purified first in this work, and followed by the modification and then adsorption and desorption of the proteins. Bovine Serum Albumin (BSA) is major material for this study because the structure is similar to the protein, which could be applied to the biosensor. The price is much cheaper than green fluorescent protein. After understanding the adsorption and desorption mechanisms and purification of BSA, the green fluorescent protein (GFP) and DsRed fluorescence protein (RFP) will be employed as major materials for the following work and developed from the simple type of biosensor device.

並列關鍵字

Montmorillonite ； Bovine Serum Albumin (BSA) ； Fluorescence protein ； Adsorption ； Desorption