- 期刊
毛細管電泳暨雷射誘發螢光於微型核醣核酸分子之檢測
Determination of MicroRNAs by Capillary Electrophoresis with Laser-induced Fluorescence
摘要
從DNA 定序到基因突變檢測，以毛細管電泳分析核醣核酸分子已經行之有年。隨著近年來生物學家陸續發現若干重要核醣核酸分子在人類生長發育及重要疾病扮演著關鍵角色，因此，針對特定核醣核酸分子而發展實用之分析方法亦成為當前重要的研究課題。在這些重要分子中，微型核醣核酸(microRNAs, miRNAs) 可說是近年來最熱門的研究主題之一；這不只因為miRNAs 是屬於內生性、有組織特異性的重要生物標幟，更特別的是此核酸分子在體液中具有一定的穩定度，而後者對於日後若要針對特定miRNAs 發展成檢驗試劑是很重要的。除了回顧科學家們發展的核醣核酸分析技術之外，本人亦將分享本實驗室最近的相關研究工作，藉此讓讀者瞭解毛細管電泳於檢測微型核醣核酸分子之應用。
並列摘要
Biologists have discovered one of the important biomarkers - microRNAs (miRNAs), and demonstrated their importance in various diseases like cancer. The field of miRNAs is currently one of the hottest topics in scientific research today. This is not only because the miRNAs are endogenous, a tissue-specific important biomarker, but also because the nucleic acid molecules in the body fluids possess a certain degree of stability; the latter is a key point in developing a clinical reagent in the future. Thus, the development of a powerful method for detecting miRNAs became an important issue for analytical chemists. From DNA sequencing to the gene mutation detection, capillary electrophoresis has been demonstrated to be effective for the analysis of nucleic acids in last two decade. The aim of this review article is to introduce recent literatures that focus on miRNAs analysis by capillary electrophoresis-based methodologies.