金奈米材料於生物感測器之發展與應用

Preparation and Applications of Gold Nanomaterials- Based Biosensors

何藺蓁(Lin-Chen Ho) ； 陳莉沂(Li-Yi Chen) ； 張煥宗(Huan-Tsung Chang)

《化學》 71卷3期 (2013/09) Pp. 233-242

https://doi.org/10.6623/chem.2013023

摘要

以金奈米材料為基礎的光學探針因其容易製備、好的靈敏度和選擇性，在生物分析領域受到很大的關注。金奈米粒子具有獨特的光學特性、高消光係數與超消光能力，可利用吸收與螢光消光等不同的方式進行光學偵測。另一方面，可發光的金奈米點則被用於分析物的螢光檢測。本篇報導簡要討論金奈米材料的製備和光學性質，除了介紹如何利用比色法和螢光檢測生物小分子、DNA和蛋白質之外，也將分析功能化金奈米粒子在不同應用策略上的優點和缺點。我們特別介紹適合體官能化的金奈米材料偵測血小板生長因子、三磷酸腺苷和凝血酶的優點。

關鍵字

金奈米粒子 ； 金奈米點 ； 生物感測器

並列摘要

Gold nanomaterials (Au NMs), including Au nanoparticles (Au NPs) and Au nanodots (Au NDs), have been extensively explored as optical probes for sensing a wide range of biological analytes such as small molecules, nucleic acids, and proteins, with advantages of stability, selectivity, and sensitivity. Various Au NPs based colorimetric and fluorescence quenching approaches have been developed by taking their size-dependent optical properties, high extinction coefficients, and/or super-quenching efficiencies. On the other hand, fluorescent Au NDs have been used for the photoluminescent detection of analytes. This review summarizes the preparation of Au NMs and their opitcal properties, and highlights the detection approaches of small molecules, DNA, and proteins. Aptamer functionalized Au NMs for the detection of PDGF, adenosine triphosphate, and thrombin are particularly emphasized to show their advantages.

並列關鍵字

Optical sensor ； Gold nanoparticles ； Gold nanodots