對蛋白質基底更靈敏方法合成螢光金奈米團簇

A More Sensitive Protein-Based Method to Synthesize Fluorescent Gold Nanoclusterss

蔡文浩(Wen-Hau Tsai) ； 黃正良(Cheng-Liang Huang) ； 陳瑞彰(Jui-Chang Chen)

《化學》 73卷3期 (2015/09) Pp. 217-224

https://doi.org/10.6623/chem.2015023

摘要

以牛血清蛋白(BSA)為基底的綠色化學常被應用於合成螢光金奈米團簇 (Au NCs)。由BSA所製備的螢光金奈米團簇經過天然聚丙烯醯胺凝膠電泳(native polyacrylamide gel electrophoresis)分析顯示，BSA聚合成為更高分子量之複合物並且與螢光金奈米團簇結合在一起。我們進一步開發以蛋白質為基礎並使用DTT作為還原劑的新合成方法，此方法可應用在BSA或其他蛋白質等具有相對高穩定性與螢光強度的螢光金奈米團簇，相較於氫氧化鈉活化方法所合成的螢光金奈米團簇，兩者具有相同激發和放射波長的螢光性質，然而，新開發的方法似乎對蛋白質偵測有相對較高的靈敏度。它僅需要較低的蛋白質濃度就可合成穩定的螢光金奈米團簇。此外低濃度牛奶也可以用此法拿來作為包覆試劑，並且成功地合成螢光金奈米團簇，顯示該方法是可以被應用在各種檢測相關蛋白樣品之潛能。

關鍵字

綠色化學 ； 牛血清蛋白 ； 螢光金奈米團簇

並列摘要

A more green chemistry has been widely used to synthesize fluorescent gold nanoclusters in the presence of bovine serum albumin (BSA). The as-prepared BSA-assisted Au clusters analyzed by native polyacrylamide gel electrophoresis indicated that BSA aggregates into higher molecular weight mass and associates with fluorescent Au clusters. A new protein-based method using DTT as reducing agent was also developed. Au clusters can be synthesized in the presence of BSA or other proteins with relatively high stability and fluorescent intensity. The product synthesized by this method exhibited similar fluorescent properties of the same excitation and emission wavelength with the NaOH-activated method. However, newly-developed method appeared to have relatively high sensitivity on protein concentration. It only required relatively low concentration of proteins to produce stable Au clusters. In addition, low concentration of milk was also used as the capping agent to successfully synthesize Au clusters, indicating that this method is capable of detecting protein related specimens.

並列關鍵字

Green chemistry ； BSA ； Nanoclusters