- 期刊
可快速偵測碳酸酐酶及篩選磺胺類藥物之開關型螢光探針
Fluorescent On/Off Switcher with Tunable Affinity for the Rapid Detection of Carbonic Anhydrase and Sulfonamide Drugs
並列摘要
We have developed a new analytical tool for the rapid and specific detection of hCAII protein in cellular lysates and various sulfonamide drugs. In this paper, three sulfonamide probes were designed and synthesized by coupling of environment-sensitive fluorophore, 4-sulfamonyl- 7-aminobenzoxadiazole (SBD) to sulfonamide ligands. One of the fluorescent probes was further applied to visualize the expression of hCAII on the cell surface of living cells. Our fluorescence turn-on strategy is based on the environment-sensitive fluorophore SBD and can be exhibit fluorescence enhancement upon the binding of the environment-sensitive fluorescent probes to hydrophobic binding domain of hCA protein. The fluorescence can be switched-off again by the addition of a ligand competing with the fluorescent probe for the protein ligand binding domain.
延伸閱讀
- 江柏毅（2014）。具可變式配體之環境敏感螢光探針對蛋白質及磺胺類藥物之選擇性檢測〔碩士論文，國立清華大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0016-2912201413513135
- 陳柏廷（2009）。建立高靈敏螢光偵測系統以篩選提升類澱粉貝塔蛋白降解酵素生產之化合物〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2009.01256
- Shun, T. C. (2022). 硫磺素T的衍生物作為替代螢光探針用於偵測人類胰島類澱粉蛋白的聚集 [master's thesis, National Taiwan Normal University]. Airiti Library. https://doi.org/10.6345/NTNU202201112
- 王敬懿、吳依萱、吳劍侯（2014）。Fluorescence Derivatization Combined with Microextraction for the Determination of Trace Amines。化學，72(4)，257-267。https://doi.org/10.6623/chem.2014026
- 吳詠朋、陳相榮、陳貴通（2017）。Current Status and Development of Novel Fluorescent Probes for Analytes Detection。化學，75(1)，63-77。https://doi.org/10.6623/chem.2017006