This paper mainly to study the establishment and influence of Pingtung Hospital, especially in the active role of public health Pingtung Hospital played. This paper discovered that as the first modem medical organization, Pingtung Hospital's establishment reasons had much to do with the polities of health and epidemic prevention. After that, it contributed a lot to preventing people's smoking opium and training nursing staff. Moreover, after World War II, part of Taiwanese staff who used to worked in Pingtung Hospital became key part of medical treatment in Pingtung. In other words, Pingtune Hospital can be called a vital organization of fostering the top-rank medical staff in Pingtung after War Word II.