掩埋場滲出水中有機成分之Ames致突變性的測驗

Mutagenicity study of Organics from landfill leachate extracts by Ames test

蘇弘毅(H. Y. Shu) ； 吳玉琛(Y. S. Wu) ； 林佩君(P. G. Lin)

《弘光學報》 40期 (2002/11) Pp. 65-71

https://doi.org/10.6615/HAR.200211.40.08

摘要

台灣地區的垃圾處理方式一直以來是以掩埋為主，而掩埋場所產生之二次污染則以滲出水問題為各封閉或掩埋中掩埋場之最大問題。本研究就掩埋場所採樣之滲出水樣品進行Ames致突變性測試，希望能了解未經適當處理之滲出水流在佈於環境中，對於生態及人體健康發生何種影響。研究中利用固相萃取法(SPE)、總固體物測定法(TS)2種不同之前處理方法，其得到的有機物再進行Ames致突變性測試，Ames的測試利用TA98、TA100二種菌種，每種樣品均分別加老鼠肝臟微粒活化酵素(S9 mix)和加S9的測試。由此研究中發現3種垃圾滲出水之SPE萃取物在經過肝臟的代謝後，均有可能對人體造成基因的段突變衝擊，但掩埋齡10~15年，掩埋物為一般廢棄物的垃圾滲出水，經一年後，它對人體基因的段突變衝擊有明顯的降低現象；掩埋齡3~4年，掩埋物除為一般廢棄物，另外還掩埋焚化爐灰渣的滲出水，在經上述2種前處理後，它不須經肝臟代謝作用即有 可能對我們人體造成基因的段突變衝擊，經一年後測試，發現它對人體基因的段突變衝擊也有明顯的降低現象。

關鍵字

滲出水 ； 固相萃取法（SPE） ； 總固體物測定（TS） ； Ames致突變性測試

並列摘要

In Taiwan, landfill was the most popular solid waste disposal method. However, the landfill leachate may cause further environmental pollution problems. The biological treatment was traditionally employed to deal with landfill leachate before discharging into surface water body. Frequently, the leachate property (water quality and quantity) varied significantly so that the biological treatment operation was not effective. Therefore, the effluent from the biological treatment failed to comply with the national discharge standards frequently. Thus, it may damage the environment and human health. In order to identify the mutagenic effect of leachate, the extracts from solid phase extraction (SPE) procedure and total solids (TS) were collected for Ames Salmonella assay. The mutagenicity of landfill leachate was examined with Salmonella Typhimurium TA98 and TA100 in presence or absence of S9 mix. The SPE extracts of leachate samples from three landfill sites showed the significant direct and indirect mutagenicity with strain TA98 and TA100. However, one year later, the same landfill leachate was collected from municipal solid waste landfill with age from 10 to 15 years and tested again. The impact of direct and indirect acting frameshift mutagens from leachate extracts decreased significantly. Furthermore, leachate from landfill with 3 to 4 years landfill age and received both municipal and incinerator ash, showed highly mutagenic to strain TA98 and TA100 without metabolic activation. Therefore, the result implied the presence of some specific direct acting frameshift mutagens.

並列關鍵字

leachate ； toxicity ； mutagenicity ； Ames test

參考文獻

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