三種美白劑的酪胺酸酶抑制動力學研究

Inhibitory Kinetics of Three Skin-Whitening Agents on Tyrosinase Activity

邱嘉玲(Chia-Ling Chiu) ； 徐照程(Jaw-Cherng Hsu)

《弘光學報》 54期 (2008/12) Pp. 115-125

https://doi.org/10.6615/HAR.200812.54.10

摘要

本研究採用酵素動力學法，探討α-Arbutin、β-Arbutin與AA-2G三種皮膚美白劑對酪胺酸酶活性的抑制機轉與能力。由動力學實驗結果得知：三種美白劑皆會降低酪胺酸酶與酪胺酸之間的親和力，α-Arbutin的Michaelis-Menten常數Km(0.275 mM)大於β-Arbutin(0.155 mM)，AA-2G(0.146mM)及無美白抑制劑存在之K_m值(0.076 mM)，較大的K_m值，代表α-Arbutin較強之酪胺酸酶活性抑制能力，此傾向亦與抑制50%酵素活性之濃度(IC_(50))的結果(α-Arbutin(1.09 mM)＜β-Arbutin(13.3mM)＜AA-2G(17.6 mM))，以及酵素抑制劑複合物(EI)之解離常數(Ki)的研究結果(α-Arbutin(0.039mM)＜β-Arbutin(0.051 mM))相符。另進一步由Lineweaver-Burk雙倒數圖形與最高反應速率(V_(max))的結果分析，可判斷α-Arbutin與β-Arbutin 傾向競爭型抑制機轉，AA-2G則傾向為混合型抑制機轉。

關鍵字

酵素動力學 ； 酪胺酸酶 ； Lineweaver-Burk圖 ； 維生素C葡萄醣苷 ； 熊果素 ； 美白

並列摘要

Inhibitory effect and kinetic behavior of three kinds of skin-whitening agent, including α-Arbutin, β-Arbutin and AA-2G, against tyrosinase have been discussed in this studies. Results showed that three kinds of the whitening agents are able to decrease the appetence between Tyrosinase and Tyrosine. The Michaelis-Menten constant Km of α-Arbutin( 0.275 mM) exceeds β-Arbutin (0.155 mM), AA-2G (0.146 mM) and the Km index without whitening inhibitor (0.076 mM). The higher Km index of α-Arbutin represents the stronger inhibitory effects, and the same tendency of inhibition potency is also shown with the results of half maximal inhibitory concentration(IC50) (α-Arbutin(1.09 mM)＜ β-Arbutin(13.3 mM) ＜ AA-2G(17.6 mM)) and dissociation constant of enzyme-inhibitor complex(Ki):α-Arbutin(0.039 mM)＜ β-Arbutin(0.051 mM). We also evaluated the result of Lineweaver-Burk plot and Vmax variation, speculated that α-Arbutin and β-Arbutin behaved as competitive-type inhibitors, and AA-2G behaved as a mixed-type inhibitor toward tyrosinase.

並列關鍵字

Enzyme kinetics ； Tyrosinase ； Lineweaver-Burk plot ； Ascorbic acid 2-glucoside ； Arbutin ； Skinwhitening agent

參考文獻

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