This article explored the nursing experience of taking care of an open tuberculosis patient admitted to a negative-pressured isolation room who faced physical and psychological challenges. During hospitalization the author used Gordon’s 11-item nursing assessment of healthy functional styles and identified the nursing diagnoses including change of nutrition status/ less than body need, knowledge deficiency, anxiety, sleep pattern disturbance, and so on. From October 4 to October 20, 2006, the author help patient accept the truth of being infected with open pulmonary tuberculosis by observation, listening, and application of empathy. As a consequent, the patient agreed to enter the negative-pressured ward. In addition to resolving his physical and psychological problems, the author also built related knowledge of his illness, made him adhere to medication, and hence completed the treatment. This nursing experience may serve as a reference for nurses while taking care of patients with pulmonary tuberculosis patient.