山豆根(Sophora tonkinensis;Shan-Dou-Gan)在傳統醫療上對老鼠腹水癌、肉瘤及子宮頸癌等腫瘤具有抑制作用。本研究探討山豆根水溶液(簡稱STAE)對老鼠淋巴癌細胞MOPC-315、MPC-11及老鼠脾臟細胞等細胞的凋亡作用及其免疫調節功能影響。結果顯示:(1)利用MTT試驗分析,STAE對MOPC-315(1×10^6/ml)的50%胞殺濃度為1.07±0.02μg/ml。(2)利用TUNEL (Terminaldeoxynucleotidyl transferase-mediated dUTP nick-end labeling)試驗及流式細胞儀(Flow cytometer)分析,證明STAE具有誘發MOPC-315老鼠淋巴癌細胞凋亡的能力。(3)連續餵食3天不同濃度STAE(25, 50, and 100mg/天)之老鼠脾臟細胞,進行RT-PCR及酵素免疫分析法(ELISA)檢測,發現STAE能促進IL-2、TNF-α及IFN-γ等T_(H1)型細胞激素之mRNA表現與蛋白質的分泌。總而言之,本研究證實老鼠淋巴癌細胞MOPC-315在STAE作用下會發生凋亡現象,藉以達到殺死癌瘤之目的;同時證實STAE具有增強老鼠抗癌免疫力之功效。
Sophora tonkinensis (Shan-Dou-Gan), used in Chinese medicine, has received a great deal of attention traditionally to antineoplastic on ascitic carcinoma, sarcoma and cervix carcinoma. In this study, we investigated the influence of the aqueous extract of Sophora tonkinensis (STAE) on mouse lymphoma cell lines, MOPC-315 and MPC-11, and lymphocytes for apoptotic analysis. The effect of STAE-fed on T_(H1)-type cytokine patterns in mice were determined by ELISA and RT-PCR. We have demonstrated that the CD_(50) of STAE for 1x10^6 MOPC-315 cells was 1.07±0.02μg/ml by MTT assay. A dose dependent induction of DNA fragmentation in MOPC-315 cells was treated with STAE using TUNEL assay. Then, data had shown that the IL-2, IFN-γ and TNF-α mRNA and protein expressions of spleen cells of STAE-3days-fed mouse were increased in a dose dependent. In conclusion, our findings suggest that STAE could induce the apoptosis of mouse lymphoma, and contained immune modulators for promoting the anti-lymphoma properties in vivo.