Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure

Farhad Ghamari ； Abdulrahman Bahrami ； Yadollah Yamini ； Farshid Ghorbani Shahna ； Abbas Moghimbeigi

《Analytical Chemistry Insights》 2016 (2016/12) Pp. 65-71

https://doi.org/10.4137/ACI.S40177

摘要

For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography-ultraviolet was used to extract trans,trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were optimized as follows: the volume of sample solution was 11 mL with pH 2, liquid membrane containing dihexyl ether as the supporter, 15% (w/v) of trioctylphosphine oxide as the carrier, the time of extraction was 120 minutes, and stirring rate was 500 rpm. Organic phase impregnated in the pores of a hollow fiber was extracted into 24 μL solution of 0.05 mol L^(-1) Na_2CO_3 located inside the lumen of the fiber. Under optimized conditions, a high enrichment factor of 153-182 folds, relative recovery of 83%-92%, and detection limit of 0.001 μg mL^(-1) were obtained. The method was successfully applied to the analysis of ttMA in real urine samples.

關鍵字

trans,trans-muconic acid ； benzene exposure ； carrier-mediated hollow-fiber liquid-phase microextraction ； HPLC-UV ； human urine