Diagnostic Implication and Clinical Relevance of Ancillary Techniques in Clinical Pathology Practice

Jaafar S. Makki

《Clinical Medicine Insights：Pathology》 2016 (2016/12) Pp. 5-11

https://doi.org/10.4137/CPath.S32784

摘要

Hematoxylin-eosin-stained slide preparation is one of the most durable techniques in medicine history, which has remained unchanged since implemented. It allows an accurate microscopic diagnosis of the vast majority of tissue samples. In many circumstances, this technique cannot answer all the questions posed at the initial diagnostic level. The pathologist has always been looking for additional ancillary techniques to answer pending questions. In our daily histopathology practice, we referred to those techniques as special stains, but nowadays, they are more than stains and are collectively called ancillary tests. They include a wide range of techniques starting from histochemical stains and ending in one or more advanced techniques, such as immunohistochemistry, immunofluorescence, molecular studies, cytogenetic studies, electron microscopy, flow cytometry, and polymerase chain reaction.

關鍵字

ancillary technique of pathology ； special stain ； histochemical stain ； molecular studies ； IHC ； immunofluorescent staining ； FISH ； cytogenetic studies ； flow cytometry ； PCR ； single-cell suspension