- 期刊
Inactivation of α_2-macroglobulin by activated human polymorphonuclear leukocytes
摘要
THE proteolytic activity of trypsin releases the dye Remazol Brilliant Blue from its high molecular weight substrate, the skin powder (Hide Powder Azure, Sigma), with an increase in absorbance at 595 nm. Active α_2-macroglobulin (80 μg/ml) totally inhibits the proteolytic activity of trypsin (14 μg/ml) by trapping this protease. But after a 20 min incubation of α_2-macroglobulin at 37°C with 2 × 10^6 human polymorphonuclear leukocytes activated by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (10^(-7) M) and cytochalasin B (10^(-8) M), 100% of trypsin activity was recovered, indicating a total inactivation of α_2-macroglobuHn. Incubation with granulocyte myeloperoxidase also inactivates α_2-macroglobulin. Hypochlorous acid, a by-product of myeloperoxidase activity, at a concentration of 10^(-7) M also inactivates α_2-macroglobulin, which indicates that an important cause of α_2-macroglobulin inactivation by activated polymorphonuclear leukocytes could be the activity of myeloperoxidase.
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延伸閱讀
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