Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules

Noriko Ito ； Yuai Li ； Tsuneo Suzuki ； Daniel J. Stechschulte ； Kottarappat N. Dileepan

《Mediators of Inflammation》 1998卷 (1998/12) Pp. 397-407

https://doi.org/10.1080/09629359890776

摘要

THE exposure of the macrophage cell line , J774 to mast cell granules (MCG) led to the formation of altered nuclear transcription factor proteins (NFkBx ), which had faster electrophoretic mobility than the p50 hom odimer of NF-k B, but retained comparable DNA binding capacity. Antibodies to N-terminal peptide s of p50, p52, p65 or c-Rel supe rshifted only a fraction of NF-k Bx. Western blot analyses revealed that nuclear p65 and c-Rel were progressively de graded after exposure to MCG, whereas nuclear p50 appeared to be unaffected. In contrast, cytoplasmic p50, p65, c-Rel as well as IkBa remained intact after MCG treatment, although p52 was clearly degraded. In comparison to J774 cells , incubation of mouse peritoneal macrophage s with MCG resulted in more extensive alterations to NF-k B proteins . The alterations in NF-k B proteins did not affect the expression of inducible nitric oxide synthase (iNOS) or TNF-a mRNA in J774 cells. These data indicate that exposure of J774 cells to MCG leads to gene ration of altered nuclear p52, p65 and c-Rel, which retain intact N-terminal peptides, specific oligonucleotide binding and transactivating activity. On the other hand, in peritoneal macrophages , MCG in duce mor e ex tens ive modifications to NF-k B proteins with associated inhibition of iNOS or TNF-a mRNA expression.

關鍵字

Macrophages ； Mast cells ； Mast cell granules ； NF-k B ； Nitric oxide synthase ； TNF-α