Zymosan-induced luminoldependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis

Haseeb Ahmad Khan

《Mediators of Inflammation》 2004卷 (2004/12) Pp. 123-125

https://doi.org/10.1080/09629350410001688558

摘要

This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 ml of reaction mixture (4x10^(-4) M luminal+50 μg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37℃. Fecal peritonitis caused a significant leukocytopenia (3540±297 mm^(-3) versus control value of 7525±711 mm^(-3), p＜0.001) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700±4006 versus 7325±425 mm^(-3), p＜0.001). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies.

關鍵字

Septicemia ； Peritonitis ； Leukocytes ； Phagocytosis ； Chemiluminescence ； Rat