Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

Olena Zakharchenko ； Christina Greenwood ； Louise Alldridge ； Serhiy Souchelnytskyi

《Breast Cancer: Basic and Clinical Research》 2011期 (2011/12) Pp. 37-42

https://doi.org/10.4137/BCBCR.S6263

並列摘要

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.

並列關鍵字

2D-GE ； breast tissue ； sample preparation ； SBO4 buffer