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Insecticides and Bio-insecticides Modulate the Glutathione-related Antioxidant Defense System of Cowpea Storage Bruchid (Callosobruchus maculatus)
並列摘要
The possible cellular involvements of cowpea storage bruchid (Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae]) glutathione and its related enzymes system in the cellular defense against insecticides (Cypermethrin and λ-cyhalothrin) and bio-insecticides (ethanolic extract of Tithonia diversifolia, Cyperus rotundus, Hyptis suavolens leaves, and Jatropha curcas seed) were investigated. The results showed that the effect of insecticides and bio-insecticides on the C. maculatus is a function of oxidative and nitrosative stresses generated in vivo. A significant (p ＜ 0.05) increase in carbonyl protein (CP) and lipid peroxidation (LPO) contents in bio-insecticides and insecticides exposed groups compared to the control indicates the extent of vital organs damage. These stresses caused similar and significant increase of glutathione peroxidase and glutathione synthetase in response to insecticides and bio-insecticide exposure in a dose-dependent manner. There was no post-translational modification of glutathione transferases expression induced. The alterations of the insect glutathione-dependent antioxidant enzyme activities reflect the presence of a functional defense mechanism against the oxidative and nitrosative stress and are related firmly to the glutathione demands and metabolism but appear inadequate by the significant reduction in glutathione reductase (GR) activity to prevent the damages. Exogenous application of reduced glutathione (GSH), to complement the in vivo demand, could not protect against the onslaught.
延伸閱讀
- Sepahy, A. A., Ghazi, S., & Sepahy, M. A. (2011). Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste. Enzyme Research, 2011(), 613-621. https://doi.org/10.4061/2011/593624
- 徐葭蓁（2013）。Studies on the Hydrolytic Enzymes of Isoflavone in Soybean:Ⅰ: Molecular Cloning and Characterization of Glycine max β-Glucosidase that Serves to Deglucosylate Isoflavone GlucosidesⅡ: Isolation and Purification of Isoflavone 7-O-Glucoside-6”- malonate Malonylesterase in Soybean〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2013.01594
- Chang, M. L., Liao, L. J., Lin, J. H., Liu, Z. H., Hsu, Y. T., & Lee, T. M. (2012). Modulation of Antioxidant Defense System and NADPH Oxidase in Pluchea Indica Leaves by Water Deficit Stress. Botanical Studies, 53(1), 45-54. https://www.airitilibrary.com/Article/Detail?DocID=1817406X-201201-201203050011-201203050011-46-55
- Cheng, E. Y., Chou, T. M., & Kao, C. H. (1984). Insecticide Resistance Study in Plutella xylostella (L.) Ⅴ. The Induction, Cross Resistance and Glutathione-S-transferase in Relation to Mevinphos-resistance. 中華農業研究, 33(1), 73-80. https://doi.org/10.29951/JARC.198403.0010
- 余卿華、楊永裕（2018）。Insecticides resistance and biochemical resistance mechanism to cyhalothrin and cartap in rice leaf folder Cnaphalocrocis medinalis。植物醫學，60(2)，15-22。https://doi.org/10.6716/JPM.201806_60(2).0003