Fluorescent in Situ Hybridization Evaluation of Epidermal Growth Factor Receptor and Cyclin D1 Genes in Oral Squamous Cell Carcinoma

Muhanad Lebnan Alshami ； Ahlam Hameed Majeed

《Journal of pioneering medical sciences》 6卷1期 (2016/03) Pp. 1-5

並列摘要

BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity, and one of the tenth most common causes of death. Although there are advances in both surgical and non-surgical treatments of oral cancer, the overall survival and mortality rates have not improved. Therefore, it is necessary to define molecular markers to allow early diagnosis, and to identify new therapeutic targets. Epidermal growth factor receptor (EGFR) is a transmembrane protein kinase which consists of extra and intracellular domains. Activation of the EGFR pathway promotes tumor cell proliferation, angiogenesis, metastasis and decreases apoptosis. Cyclin D1 (CCND1) regulates G1 to S phase transition of cell cycle. Its overexpression may lead to disturbance in the normal cell cycle control and tumor formation. METHODS: Thirty formalin-fixed paraffinembedded tissue blocks of OSCC were included in this study. Diagnostic confirmation was performed through an examination of hematoxylin and eosin (H&E) sections. Fluorescent in situ hybridization method was used to evaluate EGFR and CCND1 gene copy number. RESULTS: Fluorescent in situ hybridization evaluation showed that the EGFR amplification was present in 70% of the cases while CCND1 amplification was present in 43% of the cases. Statistically there was no significant correlation with clinic pathological findings. CONCLUSION: We did not find a statistically significant relationship between clinic pathological data and gene amplification.

並列關鍵字

EGFR ； CCND1 ； Oral Squamous Cell Carcinoma ； Fluorescent in Situ Hybridization