Globalization has made second language acquisition much more dynamic with each passing day. Learning Chinese has become one of the most active secondlanguage- acquisition activities all over the world. Lots of studies have shown difficulties English native speakers encounter when they learn wh-question formation in Chinese. Most studies provide off-line evidence to explain these difficulties native English speakers face in learning wh-question formation in Chinese. Few studies offer on-line evidence to explain this phenomenon. Therefore, the current study has adopted both on-line and off-line tasks to investigate this issue. Accuracy rate, reaction time, and acceptability rate are analyzed in the hope to offer evidence and explanations to these difficulties. Based on the result of the data, the current study firstly supports that L1 plays a role in the acquisition of wh-questions. Secondly, the proficiency level of the learners also plays a role. Thirdly, the number of clauses in a sentence can influence the process of Chinese wh-questions by learners. Fourthly, the movement of different argument roles can be a factor contributing to the difficulties in learning the whquestions.