This study implements a virtual reality (VR) environment which is based on the features of Taipei tree frog (TTF) Ecosystem, meanwhile, some issues of the sense of presence of a VR ecosystem is discussed at the end of this paper. There are two research goals in this study, first is constructing and arranging the 3D elements where a TTF lives. With the assistance of VR headsets, users may act and explore the ecological environment as a TTF; Second is to discuss the sense of presence in a VR environment. The results of this paper could support researchers to implement a VR environments and improve users’ sense of presents of a VR environment in the future.