對賽普護汰寧不同感受性草莓灰黴病菌之生理與生化特性

草莓灰黴病菌為高風險易產生抗藥性的植物病原真菌，賽普護汰寧（cyprodinil +fludioxonil）則是國內外常用於防治灰黴病的殺菌劑。供試12株草莓灰黴病菌對賽普護汰寧具不同感受性，GX2菌株對藥劑感受性最低（EC_(50)=2.986μg/mL），D9菌株最高（EC_(50)=0.023μg/mL），兩者感受性差異倍數比達129.8。低感藥菌株GX2具多重生理生化特性，包括在12～28℃溫度試驗中生長速率最慢，細胞膜通透性最高及對過氧化氫最不敏感。在抗滲透壓試驗中，於添加0.8 M山梨糖醇的培養基中，多數高感藥菌株所受生長抑制率較高，但在添加0.5M氯化鉀的培養基中，高低感藥菌株間的生長抑制率未有明顯差異。在過氧化氫培養基試驗中，高感藥菌株受過氧化氫抑制程度，明顯高於低感藥菌株。供試菌株的相對電導度均隨著測定時間而漸次增加，高感藥菌株的最終相對電導度介於38～56%，低感藥菌株則為59～69%，顯示低感藥菌株的細胞膜通透性較高感藥菌株為高。供試菌株細胞的草酸含量以高感藥菌株較高，甘油含量則在高低感藥菌株間多寡互見，而胞外多糖含量在高低感藥菌株間差異亦不明顯。

關鍵字

草莓；灰黴病菌；藥劑感受性；護汰寧；生理生化特性

並列摘要

Botrytis cinerea is a necrotrophic, filamentous phytopathogen with a high risk of developing resistance to fungicides. The phenylpyrrole fungicide fludioxonil premixed with cyprodinil has been reported to be the most frequently used fungicide against B. cinerea in Taiwan. For this study, the physio-biochemical characteristics of B. cinerea isolates with different sensitivity to cyprodinil + fludioxonil were determined. The range of EC_(50) values of cyprodinil + fludioxonil of 12 selected B. cinerea isolates were from 0.023 to 2.986μg a.i./mL. In addition, the highest resistance factor (RF) was close to 130, while all other RFs were less than 50. Among the 12 tested isolates, the least sensitive isolate GX2 had multiple physio-biochemical characteristics of the most slowly mycelial growth within 12~28°C, the highest cell membrane permeability and the least sensitive to hydrogen peroxide. The more sensitive isolates were sensitive to oxidative stress of 0.05 M hydrogen peroxide and osmotic stress of 0.8 M D-sorbitol but not by 0.5 M KCl. Compared to the more sensitive isolates with more oxalate content, the less sensitive ones had an increase in cell membrane permeability, but they did not differ in terms of cellular glycerol and exopolysaccharide content.

並列關鍵字

Botrytis cinerea ； fludioxonil ； fungicide sensitivity ； physio-biochemical ； strawberry

國際替代計量

對賽普護汰寧不同感受性草莓灰黴病菌之生理與生化特性

全文下載

主題瀏覽