稻稈誘導 Trichoderma sp. 生產纖維素水解酶

Use of Rice Straw as Inducible Substrate for Production of Cellulolytic Enzymes by Trichoderma sp.

李文翔(Wen-Shiang Lee) ； 周聖炘(Sheng-Hsin Chou) ； 林志逢(Chih-Feng Lin) ； 郭家倫(Gia-Luen Guo)

《中華生質能源學會會誌》 29卷3&4期 (2010/12) Pp. 79-87

https://doi.org/10.6385/JBESC.201012.0079

摘要

為有效降低第二代生質酒精之生產成本，於纖維素酒精廠內以當地纖維素物料誘導生產纖維素水解酶可視為重要的發展策略。稻稈是目前亞洲最大宗的農業廢棄物，因此用於誘導真菌分泌纖維素水解酶亦被認為極具運用潛力。本研究成功地運用一種熱化學技術以處理稻稈，使其作為誘導物時能顯著提升Trichoderma sp.生產纖維素水解酶之能力。其中，經由雙軸擠壓及酸溶洗前處理技術製備之稻渣作為酵素誘導原料時，可獲得最高粗酵素活性值2.1 FPU/ml，與微晶纖維素Avicel PH101所誘導的活性2.6 FPU/ml相近。若以上述前處理之稻渣為受質進行酵素水解實驗，則自產纖維素水解酶具有85.7%的水解效率，略高於商業化酵素之81.7%。此結果顯示，藉由稻稈誘導纖維素水解酶之生產方法，確實具有大規模發展的潛能。

關鍵字

纖維素水解酶生產技術 ； 酵素水解 ； 稀酸水解 ； 生質酒精 ； 木質纖維原料 ； Trichoderma sp.

並列摘要

The development of technology for ＂in-house＂ cellulase production by utilizing local biomass as inducer is considered an efficacious way for reducing the cost of the secondgeneration bioethanol. Rice straw is the most abundant agricultural waste in Asia, and then believed to have potential as an inducer to stimulate secretion of cellulase from fungus. This study was successfully developed a thermo-chemical method to make rice straw advantageous to increase the cellulase activity by Trichoderma species. In this study, the twin-screw extrusion technology was employed with dilute acid to prepare rice straw for being as inducible substrate. The maximal FPase activity of crude enzyme extract was found to be 2.1 FPU/ml, which is close to a cellulase activity of 2.6 FPU/ml from Avicel PH101 as the inducer. For enzymatic hydrolysis of pretreated rice straw, the hydrolysis efficiency of cellulase from inducible rice straw as mentioned above was 85.7%, which is higher than an efficiency of 81.7% from commercial enzyme mixture. The results indicated the method for cellulase production using rice straw is promising and worthy of industrial scale-up.

並列關鍵字

Cellulase production ； enzymatic hydrolysis ； dilute-acid hydrolysis ； bioethanol ； lignocellulosic materials ； Trichoderma sp.