蘇力菌對斜紋夜蛾幼蟲之效果

THE EFFECTS OF BACILLUS THURINGIENSIS ON SPODOPTERA LITURA LARVA

蘇智勇(Chich-Yeong Su)

《中華昆蟲》 10卷1期 (1990/03) Pp. iv+49-59

https://doi.org/10.6660/TESFE.1990004

摘要

Biobit 0.24，0.12，0.08，0.06 bp／ml FP及對照（蒸餾水）處理第1齡斜紋夜蛾幼蟲的死亡率分別為95.0，98.3，97.0，81.7及3.3％；第2齡幼蟲的死亡率分別為80.0，79.3，65.0，51.7及5.0％及第3齡幼蟲的死亡率分別為20.0，30.0，10.0，23.3及0.0％。Biobit 0.60，0.30，0.20，0.15 bp／g WP及對照（蒸餾水）處理第1齡幼蟲的死亡率分別為78.3，100.0，71.7，55.0及5.0％；第2齡幼蟲的死亡率分別為73.3，60.0，63.3，13.3及0.0％；第3齡幼蟲的死亡率分別為53.3，43.3，40.0及0.0％。觀察組織病理學研究斜紋夜蛾幼蟲的中腸上皮細胞的變化而獲得證實。接種蘇力菌12小時後，中腸上皮細胞呈現許多空胞及腸腔加大；接種24小時後，上皮細胞的體積膨腫；接種36小時後，上皮細胞破裂而墜落腸腔，腸腔中並沒有發現蘇力菌胞子。

並列摘要

The mortalities of the 1st, 2nd and 3rd instar larvae of Spodoptera litura treated with Biobit 0.24, 0.12, 0.08, 0.06 bp /ml FP and control were 95.0, 98.3, 97.0, 81.7 and 3.3%; 80.0, 79.3, 65.0, 51.7 and 5%; 20.0, 30.0, 10.0, 23.3 and 0.0% respectively. For 1st, 2nd and 3rd larval instars treated with Biobit 0.60, 0.30, 0.20, 0.15 bp/g WP and control, the mortalities were 78.3, 100.0, 71.7, 55.0 and 5%; 73.3, 60.0, 63.3, 13.3 and 0.0%; and 53.3, 43.3, 40.0, 40.0 and 0.0% respectively. Histopathological studies demonstrated extensive damage to the midgut in S. litura larvae. The epithelial cells was possessed a high degree of vacuolization and lumen of midgut enlarged 12 hr after inoculation with Bacillus thuringiensis. Twenty-four hr after inoculation, the epithelial cells of midgut had become ditsended. The epithelila cells of midgut disrupted and sloughed into the gut lumen by 36 hr post-treatment. No spore of B. thuringiensis was found in the gut lumen and neither in the cells.