Tetranychus kanzawai Kishida was observed to distribute equally between inner and outer layers of mulberry shoots. Higher population of spider mite was found frequently on leaf 5 to 11, the middle part, in a shoot. Approximately 33 to 79 percent of the spider mites in a shoot distributed in this zone depending on the length of the shoot. The correlation between the number of spider mites in this zone and in a shoot is very high. It is suggested to sample T. kanzawai population on mulberry trees in this zone.