血清替代物及無血清培養液對斜紋夜蛾細胞培養之影響

Effects of Fetal Bovine Serum Replacements and Serum-free Medium to Culture a Spodoptera litura cell line

石正人(Cheng-Jen Shih) ； 張瑞珠(Jui-Chu Chang)

《中華昆蟲》 16卷3期 (1996/09) Pp. iii+177-185

https://doi.org/10.6660/TESFE.1996018

摘要

利用乳清、卵黃乳化物及脂質濃縮物等血清替代物及無血清培養液，測試對斜紋夜蛾(Spodoptera litura)細胞株生長之影響。結果除了添加乳清之試驗組具有明顯的效果外，其他卵黃乳化物及脂質濃縮物等，在培養五代後，細胞相繼停止生長。添加乳清之TNM-FH細胞培養液，無論細胞生長曲線或細胞倍增時間，均與添加血清之培養液效果相似。利用已經商品化的無血清培養液(Sf900．II)及實驗室自行調配之培養液(ISC-06)均可成功培養斜紋夜蛾細胞株。利用ISC-06培養液培養之細胞株，其細胞數最高可達1.2x10^7 cells/ml;而Sf900-II則可達1.7x10^7, cells/ml。利用ISC-06培養液培養斜紋夜蛾SL7B細胞時，若起始培養細胞密度從3x10^6 cells/ml提高至5x10^6 cells/ml時，可加快細胞生長速度。

關鍵字

斜紋夜蛾 ； 細胞株 ； 血清替代物 ； 無血清培養液

並列摘要

The purpose of this study was to evaluate the potential application of some fetal bovine serum replacements and serum-free medium for culturing Spodoptera litura cell line SL7B, whereas lipid concentrate and egg yolk emulsion failed. Both commercial serum-free media Sf900-II and newly developed serum-free medium ISC-06 were suitable for culturing SL7B cells. The maximum density of SL7B cells produced from SIC-06 and Sf900II serum-free media were 1.2×10^7 cells/ml and 1.7×10^7 cells/ml , respectively. The cell growth rate in ISC-06 serum-free medium was improved significantly as the initial cell density increased from 3×10^6 to 5×10^6 cells/ml.

並列關鍵字

Spodoptera litura ； Cell fetal bovine serum replacements ； serum-free medium