Phylogenic Relationships in the Drosophila nasuta Species Group (Diptera: Drosophilidae): A RAPD Approach

利用RAPD的方法探討輝顏果蠅Drosophila nasuta種亞群的親緣關係（雙翅目：果蠅科）

蔡顯麞(Hsien-Chang Tsai) ； 簡一治(Yi-Chih Chien)

《中華昆蟲》 18卷3期 (1998/09) Pp. ii+167-175

https://doi.org/10.6660/TESFE.1998014

摘要

Random amplified polymorphic DNA (RAPD) analysis was applied to 14 species and subspecies of the Drosophila nasuta subgroup of the D. immigrans species group, plus D. immigrans as an outgroup. Forty primers of an arbitrary nucleotide sequence plus 2 pyruvate kinase gene-specific primers were used to amplify DNA fragments from genomic DNA of the 15 genotypes. Different RAPD fragment patterns were observed for different species, even subspecies. A dendrogram of the 15 genotypes was reconstructed using UPGMA of cluster analysis of SPSS. Data from the RAPD analysis is in concordance with that from morphological diagnostic characters in the D. nasuta subgroup, especially in the relationships between D. nasuta and D. albomicans; D. sulfurigaster spp. and Taxon-I, Taxon-j, and D. pallidifrons.

關鍵字

Drosophila nasuta ； RAPD ； phylogenic relationships

並列摘要

本研究以RAPD的分折方法針對輝顏果蠅Drosophila nasuta種亞群中的14個種及亞種進行親緣關係的分析並以D. immigrans為外群。40個隨機多型性核酸引子及2個丙酮酸鹽激專一的引子來放大此15個基因型的基因組DNA。觀察得知不同的種甚至亞種均可得到不同DNA條帶模式。一個系統樹則應用SPSS套裝軟體的群聚分析程式以UP-GMA的方式建立之。從RAPD的資料顯示，其結果和以形態診斷所得結果具一致性，尤其在D. nasuta與D. albomicans之間；D. sulfurigaster亞種間；及Taxon-I, Taxon-J及D. pallidifrons之間的關係更為明顯。

並列關鍵字

輝顏果蠅 ； RAPD ； 親緣關係