Negative humor has long been used in organizations, resulting in both positive and negative outcomes. This study describes the development of the multi-item Negative Humor Questionnaire, a measure based on confirmatory factor analysis. This 13-item scale was developed to assess individual level negative humor use. Domination and denigration are two dimensions of negative humor. The findings reveal that individuals may use negative humor intentionally in an attempt to gain superiority over other individuals and denigrate them.