- 期刊
- OpenAccess
以人工飼育蜜蜂幼蟲法評估羥四環素的藥效
Effects of Oxytetracycline of Larval Honey Bee, Apis mellifera, Reared in vitro
摘要
實驗室內人工飼育西洋蜂（Apis mellifera）1日齡工蜂幼蟲至羽化成蜂的技術已經建立，羽化率為57.1%。利用此飼育技術，添加羥四環素（oxytetracycline, OTC）5-150 ppm於配方食物中，評估其對幼蟲發育的影響。結果顯示25 ppm OTC對幼蟲的生長發育與存活率並無顯著的影響，但高於此濃度則幼蟲呈現生長延遲或存活率減低的現象。再於配方食物中加入4.5×10^5 spores/ml的幼蟲芽孢桿菌（Paenibacillus larvae larvae）和0.2-25 ppm OTC，以探討OTC對美洲幼蟲病（American foulbrood, AFB）的抑病力。食物中添加OTC者，除了0.2 ppm處理組出現1%的罹病個體，1 ppm以上的處理組皆可完全抑制AFB的發生，顯示OTC確可有效防治AFB。
並列摘要
An artificial method was developed for rearing 1-day-old worker honey bee (Apis mellifera) larvae to the adult stage in the laboratory. The proportion of adult emergence was 57.1%. This method subsequently was used to study the effects of oxytetracycline (OTC) on larval growth and development. With a concentration of 25 ppm OTC in the diet, larval and postdefecation mortalities, and larval growth rates were similar to those of the controls, while doses higher than this retarded larval growth and caused higher mortality. Feeding with 0.2 ppm OTC effectively reduced larval and postdefecation mortalities of larvae inoculated with 4.5 × 10^5 spores/ml of Paenibacillus larvae larvae. But it appeared in 1% of American foulbrood (AFB) individuals. When fed 1.0 ppm OTC and spores, no additional mortalities or AFB-infected individuals were found. This reveals that a low concentration of OTC can effectively protect young larvae from P. l. larvae infection.