Use of Random Amplified Polymorphic DNA to Characterize Entomopathogenic Fungi, Nomuraea spp., Beauveria spp., and Metarhizium anisopliae var. anisopliae, from Taiwan and China

利用RAPD方法分析比較蟲生病原真菌Nomuraea spp.、Beauveria spp.和Metarhizium anisopliae var. anisopliae分離株

高穗生(Suey-Sheng Kao) ； 蔡勇勝(Yeong-Sheng Tsai) ； 楊芃(Peng-Shian Yang) ； 洪德惠(Te-Huey Hung) ； 柯俊良(Jiunn-Liang Ko)

《台灣昆蟲》 22卷2期 (2002/06) Pp. iii+125-134

https://doi.org/10.6661/TESFE.2002011

摘要

Levels of virulence of entomopathogenic fungi among the genera Beauveria, Metarhizium, and Nomuraea are widely diverse. Random amplified polymorphic DNA (RAPD) was used to differentiate a total of 38 strains of entomopathogenic fungi isolated from 20 geographic regions of Taiwan and mainland China. Fungal isolates were obtained from 15 insect species. Banding patterns were generated from 3 selected primers (OPM 12, 18, and 20). Isolates were grouped into 10 clusters according to similarity, following cluster analysis using Jeffrey's coefficients. Three distinct genotypes were observed among the 38 isolates tested. On the basis of RAPD patterns, 2 genera and 1 species were recognized, namely Beauveria, Metarhizium anisopliae var. anisopliae, and Nomuraea. Nomuraea exhibited a more conservative banding pattern than each of the other genera. RAPD markers may be useful as identification biomarkers of specific biocontrol strains in a limited geographical area.

關鍵字

Random amplified polymorphic DNA (RAPD) ； Beauveria ； Metarhizium ； Nomuraea

並列摘要

利用RAPD（random amplified polymorphic DNA）分析方法進行來自灣本地（36株）和大陸（2株）Beauveria、Metarhizium和Nomuraea三個屬五個種（Beauveria amorpha（Ba）, Beauveria bassiana（Bb）, Beauveria brongniartii（Bbr）, Nomuraea rileyi（Nr）, Nomuraea viridulus（Nv）和Metarhiziumanisopliae（Ma）共38株蟲生真菌的鑑別分析研究，以三引子OPM 12、18、20進行反應的結果分析，Beauveria、Metarhizium和Nomuraea三個屬可明顯被區隔開來，38株菌可被區分成10個clusters，但部份M. anisopliae菌株（Ma5,6,7）和N. rileyi的親緣性反較N. rileyi與N. viridulus之間為高。

並列關鍵字

random amplified polymorphic DNA（RAPD） ； Beauveria ； Metarhizium ； Nomuraea