為有效研究水棲螢火蟲黃緣螢的行為與生活環,本研究發展出一種飼養幼蟲的新方法-“碎肉飼育法”,並設計供化蛹用的裝置。此法以切碎的新鮮田螺肉餵食單隻飼養於塑膠杯的一齡幼蟲至成熟。幼蟲齡期有五到七齡;幼蟲期整體死亡率為53.75%;各齡期死亡率以一齡幼蟲最高(18.75%),三齡幼蟲最低(1.25%)。化蛹台以插花用泡棉為材料,包含一個化桶平台與頂蓋,平台上挖出V形溝槽與圓穴,並散置泡棉屑。將化蛹台置入五齡幼蟲之飼養杯中供幼蟲化蛹。離水登陸之幼蟲會利用泡棉屑製成繭而在溝槽上化蛹,或製成圓穴之頂蓋在穴中化蛹。離水階段與化蛹過程之死亡率分別為8.75%與1.25%。成蟲可在平台上成功羽化而不傷及翅鞘,羽化率達100%。飼育出之成蟲性比為1:1,其體型與野生個體間無顯著差異。本新飼養方法與自行設計化蛹台是一種探討水生螢火蟲生活環的新方法。
To study the behavior and life cycle efficiently, a new method to rear the aquatic larvae of Luciola ficta Olivier and a self-designed apparatus for pupation were developed. The method, termed "fresh meat rearing method", used crushed and freshly cut meat of the water snail Cipangopaludina chinensis (Gray) to feed the firefly larvae, which were reared individually in plastic cups. The number of instars varied between five and seven. The overall mortality was 53.75% for the larval stage. Mortality for each instar was highest in the 1^(st) (18.75%) and lowest in the 3^(rd) (1.25%). An apparatus for pupation was made of floral foam and composed of a cover and a platform which was channeled, punched and filled with foam crumbs. The apparatus was not put into the rearing cups until the larvae reach the 5^(th) instar. The landed larvae used the foam crumbs to build cocoons and pupated on the channels, or made covers and pupated within the punched holes. The mortality for climbing larvae and the pupating larvae on these apparatus was 8.75% and 1.25% respectively. The emergence rate was 100% and the adults successfully emerged without damaging their elytra. Sex ratio was 1:1 and the body size of adults was not significantly different from the wild individuals. This new rearing method and self-designed apparatus for pupation present the good approaches of life cycles for aquatic fireflies.