Background: Health literacy, defined as individual's ability to access, understand, assess, and apply health information, has been recognized as one of the most important determinants of health. However, research on health literacy among Indonesia's population is still lacking. Objective: Our aim was to adapt the short version of European health literacy questionnaire (HLS-EU-Q16) into Indonesia context and pilot the measure in college settings, encompassing the three domains of health literacy (health care, disease prevention, and health promotion). Method: This cross-sectional study used purposive sampling technique collecting data with an online survey from first year college students of Universitas Indonesia (n=373). The original questionnaire had 16 items, each of them was rated for its perceived difficulty on a 4-point Likert scale (1=very difficult, 2=difficult, 3=easy, and 4=very easy), with a possible lowest mean score of1 and a possible highest mean score of 4. The 16 items were translated into Bahasa Indonesia and went through cognitive interviews and content validity assessment. Data were analyzed for inter-item reliability, criterion validity, as well as descriptive statistic. Results: The adapted instrument has a good inter-item reliability (Cronbach's Alpha=0.87)and has a positive association with Mental Health Literacy Scale (MHLS) as expected (r=0.27, p<0.001).The average score of health literacy among all sampleswas 2.91 (SD=0.45). The health care domain of health literacy has the highestscore (M=3.00, SD=0.47), followed by the health promotion domain (M=2.91, SD=0.61) and the disease prevention domain(M=2.73, SD=0.57). Of the three multidisciplinary colleges in the Universitas Indonesia, students from the college of health sciences had the highest health literacy score (M=3.21, SD=0.35), followed by those from the college of social and humanity (M=2.85, SD=0.48), and those from the college of science and technology (M=2.91, SD=0.61). Conclusion: The adapted short version of health literacy instrument was found to be reliable and valid for first year college students in Indonesia. Intervention is still needed to improve students' literacy in disease prevention and health promotion, as well as among students from non-health disciplines.