The purpose of this study was to examine the construct validity of Academic Motivational Regulation Scale by confirmatory factor analysis and to test the factor invariance of this scale. The subjects were 1,273 students, drawn from vocational high schools in central Taiwan, divided into two groups. The first group of 606 male students was used for the development of Academic Motivational Regulation Scale and to conduct confirmatory factor analysis. This study adopted the way of comparing the model fitness of the competitive models to choose the best model. Furthermore, this study tested the reliability and validity of the best model, including item reliability, composite reliability, convergent validity and discriminant validity. At last, the other group (667 female students) was used to measure invariance of the best model to assure the stability of the factorial structure construct validity. The factor structure was a good fit for the empirical data and invariant across gender levels.